3D Printable, injectable Amyloid-based composite Hydrogel of Bovine Serum Albumin-Aloe Vera for Rapid Diabetic Wound Healing

We have synthesized a novel amyloid-based composite hydrogel consisting of bovine serum albumin (BSA) and Aloe Vera (AV) gel to utilize the medicinal properties of AV gel and circumvent its mechanical frangibility. The synthesized hydrogel demonstrated excellent porous structure, self-fluorescence, non-toxicity, and controlled rheological properties. Moreover, these hydrogels possess inherent antioxidant and antibacterial properties, which accelerate rapid healing of wounds. The in vitro wound healing capabilities of the synthesized composite hydrogel were evaluated using 3T3 fibroblast cells. Moreover, efficacy of hydrogel in accelerating chronic wound healing via collagen crosslinking was investigated via in vivo experiments using a diabetic mouse skin model. The findings indicate that the composite hydrogel when applied, promotes wound healing by inducing collagen deposition and upregulating the expression of vascular endothelial growth factor and its receptors. We have also demonstrated the feasibility of 3D printing of hydrogel, which can be tailored to treat various types of wounds.

https://doi.org/10.1039/D3TB01151H